Analysis Note

Positive ControlSK-HEP-1 concentrated conditioned medium or breast carcinoma tissue

Application

Immunoblotting (1 µg/ml)r>Paraffin Sections (2.5 µg/ml, trypsin pre-treatment required)

General description

Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in breast carcinoma tissues.

Anti-Cathepsin B (Ab-3), rabbit polyclonal, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in breast carcinoma. It is validated for Western blotting and for paraffin sections.

Purified rabbit polyclonal antibody. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.

Immunogen

purified human liver cathepsin B

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Barrett, A.J. and Kirschke, H. 1981. Methods Enzymol.80, 535.Chan, S.J., et al. Proc. Natl. Acad. Sci. USA83, 7721.Barrett, A.J. 1987. Trends Biochem. Sci.12, 193.Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 137.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Warning

Toxicity: Standard Handling (A)